if the 403 error being generated by the request to the proxy.ashx page then you probably need to turn on windows authentication for the host asp.net application.In the web.config file for your application: <system.web>
<authentication mode="Windows"/>
<authorization>
<deny users="?" />
<allow users="*" />
</authorization>
</system.web>
This will deny access to all unauthenticated requests and allow all authenticated requests. Then make sure that anonymous access is disabled from IIS on the server.If the error is being thrown from the request from the proxy to ArcGIS server, make sure the account is valid on both the instance hosting your Asp.Net application and the instance hosting ArcGIS Server.