Maybe it is coincidence but I noticed this issue late last week on my PC with my JS3.25 WAB 2.4 Apps after I was upgraded to MS365.
We are on Enterprise 10.8.1.
Maps and popups are no longer clickable in Chrome Version 89.0.4389.90 (Official Build) (64-bit).
Our workaround is directing users to Edge or Firefox. Thankfully both still work.
Almost everything in the app that is off the map is functioning including custom and OTB widgets, zoom, scroll, etc.. Double clicking on the map Zooms in but does not bring up a popup.
The only widget that is not working is Measure which needs the map.
However, EnhancedDraw is working.
I have an untouched back up version of the app and it too is not working either in Chrome.
We have one user who is able to click on the map but the attributes are using the Esri popup rather than opening in the Scheitlin PopUpPanel. I have not been able to reproduce this.
Interestingly, I do not have this issue with any of the JS4 API apps.
This week the issue has spread to others in the organization.