Are you saying you've added the world transportation service as a layer in your MXD in ArcMap, then you serve that MXD up using ArcGIS Server? If this is the case, it's better to keep your operational layers separate from the basemap and use the client application to combine the layers. Basically take out the basemap from the MXD.
If this is not the case, perhaps you can explain more on what you're trying to do.
Thanks,
Mike